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Oxford Instruments imaris 3d reconstruction images

MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative <t>3D</t> reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean <t>±</t> <t>SEM).</t> (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Imaris 3d Reconstruction Images, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+reconstruction+images/Imaris/pmc13083801-55-41-41
Average 99 stars, based on 44395 article reviews

imaris 3d reconstruction images - by Bioz Stars, 2026-10

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Images

1) Product Images from "The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair"

Article Title: The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair

Journal: Stem Cell Reports

doi: 10.1016/j.stemcr.2026.102861

MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative 3D reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean ± SEM). (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Figure Legend Snippet: MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative 3D reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean ± SEM). (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Techniques Used: Transplantation Assay, Immunohistochemistry, Staining, Immunohistochemical staining, Expressing

MES induces the multiphenotypic differentiation of NCSCs-like cells toward neurons and Schwann cells in vitro (A) Gene expression of neuronal markers NEUROD1 , MASH1 , NGN2 , and Schwann cell markers KROX20 , NCAM1 , PMP22 after 1 week of culture under the control (C), biochemical factor (BC), MES, and MES+BC conditions. n = 4 (biologically independent), mean ± SEM. (B) Confocal images showing the expression of neuronal markers (beta III tubulin [TUJ. 1] and NEUN) after 0, 1, and 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (C) Confocal images showing the expression of a neuronal marker TUJ. 1, a Schwann cell marker GALC after 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (D) Quantification of axon length and GALC fluorescence intensity. n = 3 (biologically independent), mean ± SEM. (E) Confocal images and corresponding Imaris 3D reconstruction images of the cells after 4 weeks of culture under the control, BC, MES, and MES+BC conditions, the cells were fluorescently labeled by TUJ. 1 and GALC. NCSC-like cells were subjected to acoustic actuator stimulation as MES, biochemical factor NRG1 stimulation as BC, or the combination of both as MES+BC. The cells were stimulated for 2 h daily for either 2 weeks or 4 weeks ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01, respectively. ∗ and ∗∗ denote statistical significance p < 0.05 and p < 0.01, respectively.

Figure Legend Snippet: MES induces the multiphenotypic differentiation of NCSCs-like cells toward neurons and Schwann cells in vitro (A) Gene expression of neuronal markers NEUROD1 , MASH1 , NGN2 , and Schwann cell markers KROX20 , NCAM1 , PMP22 after 1 week of culture under the control (C), biochemical factor (BC), MES, and MES+BC conditions. n = 4 (biologically independent), mean ± SEM. (B) Confocal images showing the expression of neuronal markers (beta III tubulin [TUJ. 1] and NEUN) after 0, 1, and 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (C) Confocal images showing the expression of a neuronal marker TUJ. 1, a Schwann cell marker GALC after 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (D) Quantification of axon length and GALC fluorescence intensity. n = 3 (biologically independent), mean ± SEM. (E) Confocal images and corresponding Imaris 3D reconstruction images of the cells after 4 weeks of culture under the control, BC, MES, and MES+BC conditions, the cells were fluorescently labeled by TUJ. 1 and GALC. NCSC-like cells were subjected to acoustic actuator stimulation as MES, biochemical factor NRG1 stimulation as BC, or the combination of both as MES+BC. The cells were stimulated for 2 h daily for either 2 weeks or 4 weeks ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01, respectively. ∗ and ∗∗ denote statistical significance p < 0.05 and p < 0.01, respectively.

Techniques Used: In Vitro, Gene Expression, Control, Expressing, Marker, Fluorescence, Labeling

Software:

Article Title: Reprogramming nucleolar size by genetic perturbation of the extranuclear Rab GTPases Ypt6 and Ypt32.
Article Snippet: .. Scale bar: 1 lm. (B and C) Values of nuclear volume and surface area of the nuclear kinesin null mutants were obtained from the 3D reconstruction images using the Imaris software and were incorporated in the GraphPad Prism software. ..

Article Title: Reprogramming nucleolar size by genetic perturbation of the extranuclear Rab GTPases Ypt6 and Ypt32.
Article Snippet: .. Scale bar: 1 lm. (B and C) Values of nucleolar volume and surface area were obtained from the 3D reconstruction images using the Imaris software and were incorporated in the Graph pad Prism software. ..

Article Title: Reprogramming nucleolar size by genetic perturbation of the extranuclear Rab GTPases Ypt6 and Ypt32.
Article Snippet: .. The error bar represents SEM. (D and E) Values of nuclear volume and surface area as obtained from the 3D reconstruction images using the Imaris software and incorporated in the Graph pad Prism software. ..

Article Title: Endothelial PD‐1 Regulates Vascular Homeostasis and Oligodendrogenesis during Brain Development
Article Snippet: Images were captured using a Leica Stellaris confocal microscope and processed and analyzed using ImageJ and Prism 8 software. .. 3D reconstruction images were generated the Imaris 9.0.1 software. ..

Generated:

Article Title: FOXP Genes Regulate Purkinje Cell Diversity in Cerebellar Development and Evolution
Article Snippet: Samples were incubated at 4°C in the following solutions: 30% TEA, 40% formamide, and 30% H 2 O for 1 h; 60% TEA, 25% formamide, and 15% H 2 O for at least 4 h; and 70% TEA, 15% formamide, and 15% H 2 O for 2 h. For imaging and storage, the samples were immersed in 80% glycerol with the refractive index adjusted to 1.45, compatible with the Zeiss lightsheet Z1 microscope (5x objective). .. 3D reconstruction images and movies were generated using Imaris 9.0 (Bitplane). .. To measure the volume of flocculus, intensity-based surface objects were generated by manually outlining the structure using the surface creation module in Imaris 9.0 (Bitplane).

Microscopy:

Article Title: Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryos
Article Snippet: .. Place the PI-stained whole embryos sequentially in 30%, 50%, and 70% v/v tert-Butanol delipidation solutions (diluted with ddH 2 O) for 1–2 days. b. Immerse the embryos in the BB-PEG medium at 37°C for at least 1 day for clearing. c. Place the cleared embryos into the small pore formed by a 3% low melting point agarose on the groove of the glass slide. . d. Acquire whole-embryo fluorescent images with Leica TCS SP8 DIVE two-photon microscopy. e. Generate 3D reconstruction images by Imaris 9.0.1. i. .. Open the Imaris software (Version 9.0.1) and convert the fluorescent image files to Imaris file format using the Imaris file format converter. ii.

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